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KMID : 0895820090190040019
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Journal of Oriental Rehabilitation Medicine
2009 Volume.19 No. 4 p.19 ~ p.35
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The Effect of KangwhalSokdan-tang(Jianghuoxuduan-tang ) Administration on Muscle Regeneration in Atrophied Rat Muscle Induced by Hindlimb Suspension
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Soh Kab-Seog
Park Young-Hoi
Oh Jae-Keun
Lee Myeong-Jong
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Abstract
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Objectives: This experimental study was designed to investigate the effect of KangwhalSokdan-tang(Jianghuoxuduan-tang)on the muscle regeneration of atrophied rat muscle by hindlimb suspension.
Materials and Methods: In this study, Sprague-Dawley rats weighing about 250g were subjected to hindlimb suspension and divided into total four groups: Normal group(n=6), Control group (n=6), Hindlimb non-treatment group (n=6), Hindlimb treatment group (n=6). Experiments were seperately tried two times. The first trial was studied by the following two groups; The first was normal group (n=6). The second was group (n=18) for hindlimb suspension during 2 weeks (controlIgroup). The second trial after 2 weeks hindlimb suspension was studied by the following three groups; The third group (n=6)was expired immediately after 2 weeks hindlimb suspension. The forth group (n=6 ) was given free activity during 2 weeks after 2 weeks hindlimb suspension. The fifth group (n=6) was administrated of KST during 2 weeks after 2 weeks hindlimb suspension. In order to investigate degree of muscle atrophy, body weight and gastrocnemius muscle mass were compared. To analyze muscle regeneration factors (expression of IGF-1, Myogenin, MyoD), Western blot was used.
Results: The results were analyzed by statistical process as follows, 1. In body weight, all hindlimb suspension groups were lower than normal group, but tendency of increase was shown in KST group compared to non treatment group after 2 weeks hindlimb suspension. 2 .In gastrocnemius muscle mass, KST group on both side was significantly higher than non-treatment group after 2 weeks hindlimb suspension. 3. Incase of IGF-I, Type I of KST group was significantly increased than non-treatment group, but Type II was not shown significance. 4. There was no significantly difference in Myogenin. 5 .In MyoD, Type I of KST group was significantly increased than control group, and Type II of KST group was significantly increased than non-treatmentgroup.
Conclusions: In summary, this study demonstrates that KST administration has an effect to prevent muscle atrophy and contribute muscle regeneration and proliferation. And also it is suggested that IGF-I and MyoD is major factors of myogenesis expression to KST adminstration after hindlimb suspension.
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KEYWORD
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KangwhalSokdan-tang(Jianghuoxuduan-tang), Muscleatrophy, Muscleregeneration, Hindlimbsuspension, IGF-Ⅰ, Myogenin, Myo
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